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近红外光谱技术测定水稻直链淀粉含量研究

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AgrCopyriculiturght@201al Science&Technol 7,Information ogy,201Institute  7,18(of HAAS.Al4):729-732 l rights resewed Storage and Processing Determination of Rice Amylose Content by Near-— i nfrared SpeCtroSCopy Yumei PENG ,Huaqiang LIU,Xuedong ZHU,Xia YANG,Xiaorong HE,Huan JIANG,Xiaosuo KUANG Yudongnan Academy of Agricultural Sciences,Fuling 408000,China 近红外光谱技术测定水稻直链 淀粉含量研究 彭玉梅 ,刘华强,朱学栋,杨霞,何晓容,蒋欢, 况小锁 (重庆市渝东南农业科学院.重庆涪 陵408000) 摘 要 以298份水稻种子为样本,用常规法 测定直链淀粉含量,采用偏@,'1 ̄---乘 ̄(PLS), 优化建立精米直链淀粉含量近红外光谱预测 校正模型。模型校正决定系数RC为0.95;校正 标准差SEC为1.58:内部交叉检验决定系数 RP为0.91。标准误差SEP为1.92。利用20个 样品进行外部检验.预测值与真实值之间差异 不显著,其相关系数达95%以上。定标模型预 测性能较好,可以替代化学分析法快速测定水 稻直链淀粉含量。 China where 2,3 of the population frared light refers to electromagnetic 关键词水稻;直链淀粉含量:近红外光谱:偏 takes rice as staple food.With the im- wave with wavelength between visible 最小二乘法 provement of people’s living standard.1ight and mid-infrared light.and its rice quality has attracted more and wavelength is in the range of 780 nm— more attention.and the key emphasis 2 526 nm.The spectrum obtained by in rice breeding has been changed difuse reflection using doubled—fre— frOm yield breeding to quality breeding. quency and combined—frequency ab- Amylose content is one of the impor— sorption of H.containing groups such tant factors determining taste and as C—H,N—H and O-N,Le.,materiaI in- quality of rice.as well as one important formation included in near・infrared index for the evaluation of eating qual- range is mainly used for the qualitative itV of cooked rice[ . and quantitative analysis of organic The conventionaI method for the matter@].This method is safe and en. determination of amylose content Is vironmentally-friendly. belonging to standard iodine—blue colorimetry.The “green analysis”technique.and could conventionaI chemicaI method has the simultaneously determine various disadvantaqes of complicated opera— components in samples with the ad- tion,time consuming,labor consuming vantages of simple operation,high and long measurement period.and is analysis efficiency and Iow cost. quite dificult for the detection of mass ln recent years.with the continu. samples.Therefore.the requirements ous extension and application of NIR for early screening by breeding re- spectrometry technique,this technique searchers could not be satisfied,and has been widely applied in quality the research about rice breeding is breeding of diferent crops. Many hindered. scholars obtained satisfactory results 作者简介 彭王梅(1973一),女,重庆涪陵人. 农艺师,主要从事实验测试分析及近红外光谱 分析技术的相关研究,E-mail:564377718@qq. }Corresponding author;E-ma ̄:564377718@qq corn corn 通讯作者。 , Received:Decem ̄29’2016 Aocep挺Id:March25.2017 一0= 。 收稿日期2016-12—29 修回日期2017-03—25 73O 2O17 in the determination of amylose and calibration set for modeI establish. ment.and 1/3 of them were used as the validation set.The divided calibra- tion set and validation set should be u— ln this study.an optimaI mode1 was protein in crops including rice,wheat and maize . Diferent types of near.infrared established according to the principle 0f maximum calibration determination coefficient and minimum standard de. viation by selecting suitable spectral intervaI and better spectral pretreat. spectrometers have diferent perfor- mances,and there are diferent pa- rameters,calibration softwares and n|f0rm and representative.Twenty samples were reserved as a prediction set. ment method and adopting PLS and cross validation. of spectrum analysis objects。calibration results ob- Pretreatment tained in previous studies could not be cited directly.In this study.rice mated— als provided by Rice Research Insti— tute,Yudongnan Academy of Agricul— In Order to eliminate baseline drit.f background and light scattering of NIR spectraI signal and improve prediction accuracy of model,pretreatment should be performed to primary spec- Resu lts and Analysis Acquisition of primary spectrum and chemical determined values of turaI Sciences were detected by a amylose content near-infrared analyzer,a corrected model of rice amylose was established by pa ̄ial least squares(PLS),and the accuracy of the mode1 was validated. Materials and Methods Tested materials and treatment As experimental materials.298 rice samples were selected.Each sample was obtained by careful screening,the seeds were ground, and full uniform polished kernels with- out damage were selected and sub- iected to near-infrared detection. Instruments SUPNIR一2720 near-infrared spectrometer produced by Focused Photonics lnc..installed with corre— sponding analytic software as well as notebook computer;51 88 type rice polisher. Chemical determination of amylose content Amylose content was determined by Quality Inspection Center of Rice Products,Ministry of China. Establishment of Nl analysis model Scanning of original spectrum Before measurement,the near- infrared spectrometer with a wave- length range of 1 000-1 799 nm was preheated for 30 min.The sample box rotated uniformly under the difuse..re—. fiection scanning mode.During sam— pie loading,samples were loaded nat urally with hand to ensure that each sample had the same compactness, so as to reduce error.Each sample was scanned for 2 times,and average spectraI data were used in near-in- frared spectroscopic analysis. Division of sample sets The samples were ranked accord・ ing to amylose contents{rclm low to high.2,3 Of them were selected as the trum【1o】.and this treatment process is The primary spectrum of rice was performed by the analytical software shown in Fig.1.and the chemicaI de. (model management)installed on the termined values of amylose content spectrometer.Diferent spectral pre— were shown ln Table 1. treatments were compared。and It was In near-infrared spectraI analysis. finally determined that the pretreat— the number of calibration samples and ment was completed by combining first component content range affects the derivation,Savitzky—Golay smoothing, stability and accuracy of analyticaI multiplicative scatter correction and model[ .As shown in Table 1.the de— mean centering. termined amylose contents of the 298 Establishment of model rice samples were in the range of The determined values of rice 2.1 O%-28.40%.indicating that the amylose content was correlated with amylose content varied greatly,with a the spectral data of samples,quantita- larger covering range.The amylose tive analysis was performed to sam— contents of the calibration set.valida- pies by chemometry.and a mode1 was tion set and alI samples were distribut・ established.Chemometrics methods ed closely,and the validation set was commonly used in near.infrared anal— included in the calibration set.indicat- ysis are multiple regression(MLR), ing that the division of sample set was stepwise regression(SRA),principal more rationa1.The selected sample in component regression (PCR),PLS this experiment had very good repre— and artificiaI neauraI network(ANN) . sentativeness.and were suitable for Table 1 Distribution of rice amylose contents Table 2 Comparison between determined values by chemical method and predicted values by near-infrared spectroscopy % 2017 731 0.96.The slope and the correlation 1・4 coefficient were both close to 1.indi・ cating that there was a good Iinear re- lationship between chemicaI values and the predicted values.Therefore, the modeI has as a good predication 1.2 莹1-o 号0.8 兰 0.6 0.4 0.2 effect. Conclusions The near-infrared spectroscopic 善量善景莩量耄詈害oo Fig.1 Primary spectrum of rice Fig.3 Correlation between determined val— ues and predicted values in internal cross validation of near-infrared model acquisition of primary spectrum and establishment of mode1. Establishment of optimaI model Diferent principal components were selected by comparing diferent spectral pretreatment methods and analyticaI methods as welI as optimal spectraI ranges.Fig.2.the PRESS figure was the basis for selecting prin- ciple factors of mode1.and the optimal parameters of the optimized model were obtained.The number of select— ed principle factors was 1 0.the call— bration determination coefficient(RC) was 0.95.the standard error of calibra- tion(SEC)was 1.58,and the standard error of cross.validation(SECV)was 1.84.1t could be seen fr0m the model parameters that the correlation coeffi- cient was higher,the standard devia- tion was lower.and combined with SECV≤SEC+1.2 and 1.84≤1.90 (1.58+1.2),it was indicated that the modeI achieved a better effect. Validation of correction model InternaI validation Internal cross validation was per- formed to the established modeI using the automatic validation function of the software,and predication was per— formed to the samples;n the validation .2 PRESS Figure Determined va1UC by chemical method/f% Fig.4 Correlation between determined val- ues and predicted values of samples in pre- diction set set(Fig.3).The results showed higher determination coefficient RP of 0.91。 and Iower standard error of prediction SEPOf 1.92. External validation The predication set(not participat- ing in establishment of model1 includ— ed 20 rice samples,and the predica. tion effect of the established mode1 was evaluated by externaI validation method.The determined values and model--predicted values of rice amy-・ Iose content were shown in Table 2. 1t could be seen fr0m Table 2 that the absolute error values of amylose content determined by chemicaI method and spectral method were in the range of 0.O9—2_22.The chemicaI values and predicted values were sub. jected to logarithmic t test by statjsticaI software DPS7.05。giving t=0.21 and to.o6(1 9)=2.09,Le.,ltl<to.衢,which Indicat— ed that there was no significant difer- ence in determined values between the 2 methods. The correlation between the chemicaI values and the predicted values was exhibited by drawing a scatter diagram between them(Fig.4). The slope of the trend Iine was 0.94. and the correlation coefficient was analysis is a rapid.efficient and non- destructive,wo ̄hy of extension.In this study.the near-infrared modeI of rice amylose content was established by PLS.This modeI had larger correlation coefficient of 0.95 and Iower standard deviation of 1.58.Prediction was per- formed to 20 samples not participating In modeI establishment.and there was higher correlation between the chemi- caI values and the prediction values by mode1.indicating lhat the modeI had very good prediction abUity,and would be feasible for generaI determination of amylose content in rice.This study provides a new efficient determination method for rice quality breeding,which greatly improves testing efficiency,and could guide materiaIidentification and variety screening in rice breeding. There was high correlation be. tween the near-infrared analysis re. suits and chemical values of amylose content, and calibration samples should cover the change range of content of to・-be・・detected component uniformly.In this study,fewer varieties in the selected samples had the amy. Iose contents in the range of 1 5%一 20%.which certainly affected the ac. curacy of the mode1.Further collection of samples for such index could im. prove the precision of modeI and the accuracy of prediction. In determination bv near.infrared analytical method,factors including component content,sample number, physical properties of samples,pre— treatment method of samples,test condition and instrument all would af. fect the accuracy of the near-infrared analysis[馏.Therefore.the effects fr0m various factors should be taken into consideration, and corresponding prevention measures should be taken, s0 as to improve the reliabiliyt and ap. plicability of established analytical mode1. ,32 2O17 References 【1】YU F(余飞),DENG DW(邓丹雯), DONG J(董婧),et a1.Influence factors of amylose content and its applied re— XL(李晓利),eta1.Study on NIR spec— tral detection modeI of rice protein con— model for apparent amylose content of search progress(直链淀粉含量的影响 因素及其应用研究进展)『J】.Food Sci— ence(食品科学),2007,28(1O):604— 608. tent(大米蛋白质含量近红外光谱检测模 型研究)『J1.Chinese AgriculturaI Sci. ence Bulletin(中国农学通报),2013,29 (12):212-216. [6】PENG J(彭建),ZHANG ZM(张正茂). Rapid determination of starch and amy. 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